CED9在烟草中表达改变了离子流对盐胁迫和氧化胁迫的反应——植物细胞凋亡与离子流 - 植物学(Plant science) - 生命科学中的应用(Life sciences) - 美国扬格非损伤技术中心

超级版主

UID 648
精华 0
积分 435
帖子 54
阅读权限 150
注册 2008-7-21
状态离线

发表于 2009-5-13 09:43 资料短消息加为好友

CED9在烟草中表达改变了离子流对盐胁迫和氧化胁迫的反应——植物细胞凋亡与离子流

Expression of animal CED-9 anti-apoptotic gene in tobacco modiWes plasma membrane ion Xuxes in response to salinity and oxidative stress CED9在烟草中表达改变了离子流对盐胁迫和氧化胁迫的反应——植物细胞凋亡与离子流

Abstract
Apoptosis, one form of programmed cell death (PCD), plays an important role in mediating plant adaptive responses to the environment. Recent studies suggest that expression of animal anti-apoptotic genes in transgenic plants may signiWcantly improve a plant’s ability to tolerate a variety of biotic and abiotic stresses. The underlying cellular mechanisms of this process remain unexplored. In this study, we investigated speciWc ion Xux “signatures” in Nicotiana benthamiana plants transiently expressing CED-9 anti-apoptotic gene and undergoing salt- and oxidative stresses. Using a range of electrophysiological techniques, we show that expression of CED-9 increased plant salt and oxidative stress tolerance by altering K+ and H+ Xux patterns across the plasma membrane. Our data shows that PVX/CED-9 plants are capable of preventing stress-induced K+ eZux from mesophyll cells, so maintaining intracellular K+ homeostasis. We attribute these eVects to the ability of CED-9 to control at least two types of K+-permeable channels; outward-rectifying depolarization-activating K+ channels (KOR) and non-selective cation channels (NSCC). A possible scenario linking CED-9 expression and ionic relations in plant cell is suggested. To the best of our knowledge, this study is the Wrst to link “ion Xux signatures” and mechanisms involved in regulation of PCD in plants.